Barrier Cell Migration Assay
Cell exclusion zone assays, or barrier assays, rely on the creation of a cell-free zone without the use of a mechanical scratch. Removal of the temporary barrier enables surrounding cells to migrate into the open area. With this application, cell migration into the wound area over time is monitored within the Agilent BioTek Lionheart FX automated live cell imager or the Agilent BioTek Cytation cell imaging multimode reader. The Agilent BioTek Gen5 microplate reader and imager software analysis delivers detailed and reproducible cell migration results.
Label-Free Barrier Cell Migration Procedure
Step 1. Capture: Kinetic high contrast brightfield (HCBF) images our captured based on desired interval and duration.
Step 2. Analyze: Images are processed and analyzed to monitor migration into the open area.
Step 3. Report: Kinetic profiles provide detailed information on cell migration across treatment conditions.
Increase assay throughput and performance
The Agilent BioTek BioSpa automated incubator performs live cell analysis and enables multiplate kinetic analysis of the barrier cell migration assay when integrated with the Cytation live cell imager. BioSpa compatible assay performs live-cell analysis on up to 8 microplates.
Figure 1. The BioSpa live cell analysis system
Liquid handling with the Agilent BioTek MultiFlo FX multimode dispenser automates cell seeding and removes piled up cells from around cell zone for improved assay results.
Figure 2. MultiFlo FX multimode dispenser.
Application Notes:
- Imaging and Analysis of a High Density Cell Migration Assay
- Performance of a Label-Free Image-Based 2D Scratch Wound Healing Assay to monitor Cell Migration and its Inhibition
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Automated Label-free Method for Measuring Cell Migration in Real-Time with Oris Pro Assay
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For Research Use Only. Not for use in diagnostic procedures.
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