ELISA - BioTek Applications

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- Imaging & Mikroskopie
  Vollautomatisierte Cell Imager
  
  Lionheart FX Vollautomatisiertes Mikroskop
  Lionheart LX Vollautomatisiertes Mikroskop
  Reader für konfokales Imaging
  
  Cytation C10 Reader für konfokales Imaging
  Multi-Detektions-Reader für Cell Imaging
  
  Cytation 7 Multi-Detektions-Reader für Cell Imaging
  Cytation 5 Multi-Detektions-Reader für Cell Imaging
  Cytation 1 Multi-Detektions-Reader für Cell Imaging
  Multi-Plate Live Cell Imaging System
  
  BioSpa Lebendzell-Plattform
  Peripheriegeräte
  
  Cell Count & Viability Starter Kit
  AutoScratch Wound Making Tool
  Gaskontroll-Modul
  Reagenzdispenser-Modul
  Produktübersicht
  
  Imaging & Mikroskopie
  
  Gen5 Datenanalyse-Software
- Detektion
  Multi-Detektions-Reader
  
  Synergy Neo2 Multi-Detektions-Reader mit Hybrid-Technologie
  Cytation Multi-Detektions-Reader mit Hybrid-Technologie
  Synergy H1 Multi-Detektions-Reader mit Hybrid-Technologie
  Synergy HTX Multi-Detektions-Reader
  Synergy LX Multi-Detektions-Reader
  Multi-Plate Absorbance Reader
  
  LogPhase 600 Reader für Mikrobiologie
  Mikroplatten-Reader
  
  Epoch 2 Mikroplatten-Spektralphotometer
  Epoch Mikroplatten-Spektralphotometer
  800 TS Mikroplatten-Reader für Absorptionsmessungen
  Peripheriegeräte
  
  Gaskontroll-Modul
  Take3 Mikrovolumen-Platte
  Reagenzdispenser-Modul
  Produktübersicht
  
  Multi-Detektions-Reader
  
  Mikroplatten-Reader
  
  Mikroplatten-Spektralphotometer
  
  Gen5 Datenanalyse-Software
- Liquid Handling
  Washer/Dispenser
  
  EL406 Washer/Dispenser-Kombination
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  Mikroplatten-Washer
  
  405 TS Mikroplatten-Washer
  405 LS Mikroplatten-Washer
  ELx405 Mikroplatten-Washer
  50 TS Mikroplatten-Washer
  Mikroplatten-Dispenser
  
  MultiFlo FX Multimode-Dispenser
  MicroFill Mikroplatten-Dispenser
  Produktübersicht
  
  Washer
  
  Dispenser
- Automation
  Automation
  
  BioSpa 8 Vollautomatisierter Inkubator
  BioStack Mikroplatten-Stacker
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  Produktübersicht
  
  BioStack Modelle
- Software
  Software
  
  Gen5 Software für Imaging & Mikroskopie
  Gen5 Software für Detektion
  Liquid Handling Control Software
  Software Apps
  
  Scratch Assay App
  Cell Count & Viability App
  LogPhase 600 App
  Produktübersicht
  
  Gen5 Datenanalyse-Software
- Buy Cell Culture Microplates
  Sourcing your microplates shouldn't be an added task in preparing your cell-based workflows. Agilent now offers cell culture and imaging microplates so you can complete your workflows seamlessly.
  
  Click here to request your free sample.
- Technologien
  Multi-Mode Detection Technologies
  
  Absorbance
  Alpha Detection
  Filtered Luminescence
  Flash Luminescence
  Fluorescence Intensity
  Fluorescence Polarization
  Glow Luminescence
  Time Resolved Fluorescence
  TR-FRET
  Imager & Microplate Reader Technologies
  
  4-Zone Incubation and Condensation Control
  Augmented Microscopy
  Confocal Technology
  Hybrid Technology
  Laser Autofocus
  Peltier Cooling Module
  Monochromatoren mit variabler Bandbreite
  Liquid Handling Technologies
  
  AMX Technology
  Dual-Action Manifold
  Parallel Dispense
  RAD Technology
  Ultrasonic Advantage
  Verify Technology
- Angebotsanfrage
  
  Nutzen Sie bitte den folgenden Produktkonfigurator zur Auswahl passender Instrument-Modelle.
Peripheriegeräte
- Peripheriegeräte
  BioTek bietet eine Vielzahl an Peripheriegeräten und Zubehörteilen zur Erweiterung der Funktionsvielfalt und Optimierung von Arbeitsabläufen. Die Peripheriegeräte sind mit vielen verschiedenen Produkten kompatibel. Eine vollständige Übersicht finden Sie hier.
- Zubehör
  Um Ihren BioTek Imager, Mikroplatten-Reader, Liquid Handling- oder Automationssystem zu vervollständigen oder zu optimieren, bieten wir eine breite Palette an Zubehör, das von Schläuchen und Flaschen bis hin zu Imaging Filter Cubes reicht. Schauen Sie in unserer Zubehördatenbank nach Zubehör, das mit Ihrem Gerät kompatibel ist. Suchen Sie anhand von Schlagwort, Teilenummer, Produkt oder Zubehör.
- Starter Kits
  BioTeks Starter Kits enthalten alles, was Sie benötigen, um die neusten Assays schnell und nach nur kurzem Training anzuwenden. Die Kits bieten Hardware, Software, Verbrauchsmaterialien und Reagenzien in einem praktischen Set.
Applikationen
- Cancer Research
  The field of cancer research spans diverse disciplines and requires a multipronged approach to understand the complex factors driving cancer formation and progression, as well as for the development of effective therapeutics.
  
  BioTek instruments provide versatile platforms and integrated solutions for conducting a broad range of cancer research studies.
- Cell Counting
  The ability to accurately determine cell number is an important aspect of a broad range of applications, including setting up and optimizing cell-based assays, normalizing data across samples, and conducting cell proliferation assays.
  
  The Agilent BioTek Lionheart FX automated microscope and Agilent BioTek Cytation cell imaging multi-mode readers utilize label-free and fluorescence imaging modes, along with powerful Agilent BioTek Gen5 image analysis tools, to deliver efficient and effective automated cell counting.
- Cell Culture QC
  
  Automated imaging tools can provide valuable information for improving routine cell culturing techniques and increasing the effectiveness and reproducibility of downstream cell-based assays. Conventional methods for evaluating cell culturing techniques and assay design consist of manual inspection of a small subset of the cell population at random locations and time points.
- Cell Health and Viability
  
  Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. Diverse assay formats and reagents have been developed that measure specific aspects of cell viability corresponding to particular cellular response pathways and mechanisms of injury.
- Cell Metabolism
  
  Cellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. These orchestrated reactions, better described as metabolic pathways allow organisms to function and respond to environmental changes. Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis.
- Zellmigration und Invasion
  
  Cell migration and invasion are live cell kinetic assays useful in oncology research. The principle difference between migration and invasion is that migration refers to normal cell movement; whereas invasion describes cells actively invading surrounding tissue. It can do this under the influence of a chemoattractant gradient and secretion of proteases. There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models.
- Developmental Biology
  A macro trend in drug discovery is the use of complex models of disease early in preclinical studies. An approach following this viewpoint is the use of whole organism models involving living invertebrates and vertebrates such as nematodes and zebrafish conducive to drug screening methods.
- Endotoxin Analysis
  Bacterial endotoxins are lipopolysaccharides (LPS) generally found in the outer membrane of gram-negative bacteria, such as E.coli, and are widely present in our environment. Because of the pyrogenic nature of endotoxins, with human effects from fever to hemorrhagic stroke, FDA regulations require that raw materials (e.g. water) used in parenteral drugs be tested for endotoxin levels.
- Histology and Slide Scanning
  Histology is typically performed on fixed tissue samples using upright microscopes equipped with oculars. Cytation™ and Lionheart™ FX provide automated digital imaging of these samples using color brightfield microscopy. Low to high magnifications from 1.25x to 60x (100x with Lionheart FX) can be combined with Cytation Gen5™ image processing features such as image stitching to increase field of view.
- Immunology
  The immune system relies on diverse mechanisms working in concert to defend the host from infection and to identify and remove aberrant or damaged cells. Dysregulation of the immune system can lead to a variety of disease conditions, including autoimmune disorders, allergies, and cancer. Additionally, the immune system is a critical consideration for vaccine development and cell, tissue and organ transplants.
- Immuno-Oncology
  While harnessing the patient’s immune system to fight cancer is not a new concept, there have been significant strides in the past decade to more effectively direct and activate components of the immune system and counter the measures certain tumors adopt to shield itself from immune attack. These include immunotherapeutic drugs based on directing immune cells to tumors based on specific antigens they present, PD-1 checkpoint inhibition and the ex vivo manipulation of the patient’s own immune cells to better drive an immune response to cancer.
- Mikrobiologie
  Since the advent of a lens system that enabled the visualization of bacteria and protists in 1671, the microscope remains a ubiquitous tool for the scientific study of live and fixed microorganisms. Investigating the structure, function, and typing of the highly diverse array of microorganisms spans heterogeneous applications including, but not limited to, the elucidation, diagnosis, and treatment of human and plant disease, food and water safety testing, vaccine development, investigation and production of industrial lipases, protein transfection to mammalian cells, environmental health and impact appraisal, horticultural and agricultural soil assessment, and exploration of alternative fuel sources.
- Molecular Biology and Biochemical
  Biochemical assays run the gamut from routine ELISAs to micro-volume nucleic acid and protein quantification. Endpoint and kinetic, single- and multi-wavelength assays are essential experimental tools. BioTek’s multi-mode readers have the flexibility to accommodate a very broad range of applications with superior performance.
- Rapid Cell Signaling
  G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) represent large, diverse gene families found in fungi, plants and animals. The GPCR family of receptors is involved primarily with internalizing extracellular environmental signals via interaction with intracellular G-proteins. GPCR ligands and modulators range from peptide and non-peptide molecules to chemokines, nucleosides, growth factors and light.
- Stem Cell Research
  Research into the reprogramming of somatic cells into pluripotent stem cells received the Nobel Prize in 2012. The long term importance of this research resides in the use of stem cells for regenerative therapies; for the short term, however these cells represent a potential unlimited source of highly physiologically relevant cells, akin to human primary cells, that can be used as drug discovery reagents.
- Virology
  Research into the diverse aspects of virology are made possible by a broad range of applications and assay formats, from relatively simple methods for viral detection to those designed to elucidate complex immune responses and pathological processes. BioTek instruments provide versatile platforms for conducting viral research, with optimized imaging- and plate reader-based solutions, as well as liquid handling and automation tools for increased assay throughput and reproducibility.
Quellen
- Gefragte Applikationen
  
  In BioTeks Applikationshinweisen finden Sie die aktuellsten wissenschaftlichen Inhalte bezogen auf die Anwendungsmöglichkeiten unserer gesamten Produktpalette. Suchen Sie nach Themen, Detektionsmethoden und Produkten oder nutzen Sie einfach unsere Stichwortsuche, um hilfreiche Applikationshinweise zu erhalten.
- Applikations-Mitteilungen
  
  Die Applikations-Mitteilungen von BioTek enthalten kurze, prägnante Beschreibungen aktueller Anwendungen, die mit den Life Science Produkten von BioTek möglich sind.
- App Guides
  
  Viele Anwendungen sind einfach und bedürfen keiner weiteren Erklärungen. Handelt es sich jedoch um komplexe Abläufe sind Best Practice und Tipps & Tricks sehr hilfreich. Hier bietet Ihnen BioTek detaillierte Informationen zu einer Vielzahl an Themen.
- Journale/Publikationen
  Stöbern Sie in unserer Datenbank nach wissenschaftlichen Artikeln die von BioTeks Wissenschaftlern in führenden Fachzeitschriften veröffentlicht wurden.
- Poster
  In über 170 wissenschaftlichen Postern präsentiert Ihnen unser Applikationsteam seine aktuellsten Forschungsergebnisse und Applikationsmöglichkeiten unserer Life Science Produkte. Suchen Sie hier nach interessanten Themen und Produkten.
- Technische Hinweise
  
  Die technischen Hinweise veranschaulichen produktspezifische Eigenschaften und gängige Anwendungsmöglichkeiten unserer Mikroplatten-basierten Produkte in Ihrer täglichen Versuchsplanung.
- Graphische Präsentationen
  BioTeks graphische Präsentationen sind kurze, animierte Präsentationen die die Schrittabfolge bei der Verarbeitung einzelner Imaging- und Multi-Detektions-Anwendungen beschreiben.
- Webinare
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- White Papers
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- Literatur
  
  Erfahren Sie in über 3000 wissenschaftlichen Veröffentlichungen mehr über die weltweite Anwendung unserer Life Science Produkte.
- Videos
  Hier finden Sie eine kompakte Übersicht aller Videos die auf unserer Webseite veröffentlicht sind. Sie finden die Videos auch auf unserem BioTek YouTube Kanal.
- Kommende Webinare
  In unseren Webinar-Präsentationen geben wir Ihnen hilfreiche Informationen und nützliche Tipps zu den aktuellsten Mikroplatten-Anwendungen. Registrieren Sie sich bereits heute und erfahren Sie mehr über zukünftige Themen.
Technische Unterstützung
- Vertriebsbüros
  BioTek's highly trained sales and service professionals are experts in life science instrumentation. They will guide you through your decision process and help you select the proper equipment for your application, assist with installation in your lab, and train your staff on the use of the instrumentation.
- Field Applications Scientists
  BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software.
- Technische Unterstützung
  The BioTek Global Technical Support Center (GTS) is your Help Desk for all questions related to BioTek product use, maintenance, troubleshooting support and other frequently asked questions. Normal GTS hours are weekdays from 8:30 A.M. - 5:30 P.M Eastern Standard Time, excluding holidays.
- Weltweiter Service
  BioTek Instrument Service and Repair is available worldwide at several of our International Service Centers. Field Service is also available for many customers throughout the world.
- FAQ
  Search our Frequently Asked Questions by keyword, product or category. For additional assistance call (800) 242-4685 or submit a question via our Ask a Question form.
- Customer Resource Center
  BioTek's Customer Resource Center (CRC) gives customers access to information about their specific BioTek instrumentation and software. Register and log in to:
  
  - Check status and track orders
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  - Access documentation, user manuals and software
  - Request Service & Support
- Unterstützung anfordern
Über uns
- News
  Synergy Neo2 and Synergy H1 Now Available with Expanded Temperature Control Range
  July 28, 2021 - Now with temperature control up to 70 ºC, BioTek’s Synergy Neo2 Hybrid Multi-Mode Reader and Synergy H1 Hybrid Multi-Mode Reader aid in more assay workflows than ever before. Application flexibility extends to enzyme kinetics, protein stability and aggregation, and projects incorporating loop mediated isothermal amplification (LAMP) assays. Weiterlesen
  
  BioTek's Cytation 7 Cell Imaging Multi-Mode Reader Named ‘Best New Drug Discovery & Development Product’ at Scientists’ Choice Awards
  May 5, 2021 - BioTek Instruments is pleased to announce that the Cytation 7 Cell Imaging Multi-Mode Reader was awarded ‘Best New Drug Discovery & Development Product’ of 2020. The honor was voted upon by SelectScience members across the world and celebrated at the 2021 Scientists’ Choice Awards online awards ceremony. Weiterlesen
  
  BioTek Announces New Cytation C10 Confocal Imaging Reader
  February 2, 2021 - BioTek Instruments, a part of Agilent, is excited to unveil the Cytation C10, an advanced, automated Confocal Imaging Reader. This versatile and multi-functional automated system is ideal for research laboratories and core facilities looking to increase productivity while reaping high-quality qualitative and quantitative data to support cell-based research. Weiterlesen
  Weitere News
- Veranstaltungen
  LS2 Annual Meeting 2022
  
  February 16 - November 18, 2022
  
  Booth #21
  
  Irchel campus, University of Zurich
  BioTek instrumentation will be on display by BioTek Switzerland.
  
  Zurich
  Switzerland
  
  Experimental Biology 2022
  
  April 2 - November 5, 2022
  
  Booth #605
  
  Pennsylvania Convention Center
  BioTek instrumentation will be on display by BioTek U.S.
  
  Philadelphia. PA
  United States
  
  Weitere Veranstaltungen
- Customer Spotlights
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- Unsere Geschichte
  At BioTek we feel passionately and genuinely that our employees are our greatest asset. We continue to build our already impressive team having doubled our global workforce in the last five years. With our headquarters in the beautiful Green Mountains of Vermont, USA, we also have regional offices around the world.
  
  If you're interested in becoming part of our amazing team, check out our list of job openings today!
- Partner
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- Blog
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- Qualität
  BioTek fühlt sich verpflichtet die hohe Qualität unserer Produkte und Dienstleistungen zu bewahren. Alle unsere Produkte werden in unserem Firmensitz in Winooski (USA) entwickelt und hergestellt. Zu unserem fortlaufenden Verbesserungsprozess gehört die Einbindung von Kundenanregungen hinsichtlich Qualität und Eigenschaften unserer Produkte, mit dem Ziel neue leistungsfähige und zuverlässige Produkte zu entwickeln, zu validieren, herzustellen und zu testen.
- Karriere
  At BioTek we feel passionately and genuinely that our employees are our greatest asset. We continue to build our already impressive team having doubled our global workforce in the last five years. With our headquarters in the beautiful Green Mountains of Vermont, USA, we also have regional offices around the world.
  
  If you're interested in becoming part of our amazing team, check out our list of job openings today!
- Kontakt
  Contact BioTek today. Ask a sales or technical question or request service.

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The enzyme linked immunosorbent assay (ELISA) is a very common, robust technique for detecting various analytes. It spawned a broad range of immunoassays that utilize either wash or no-wash workflows using colorimetric, fluorometric, luminometric, TRF or TR-FRET, AlphaScreen and AlphaLISA detection technologies. Some of these latter technologies utilize low volume analysis in 1536-well plates, yet ELISA performed in 96-well microplates remains the most used immunoassay technique. The ELISA technique causes formation of specific immune complexes that are easily measured using the Agilent BioTek single or multimode microplate readers. The basic building blocks of an ELISA reaction are:

ELISA process

Application Notes

Technical Notes

Benchtop ELISA Automation with BenchCel Microplate Handler

Publications

Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination

Application Bulletins

Detection of HTLV I and II Antibodies using Absorbance based ELISA

Learn more about ELISA – see our applications and technical documentation in Resources.

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Agilent BioTek Cytation 7 Cell Imaging Multi-Mode Reader
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Agilent BioTek Synergy Neo2 Hybrid Multi-Mode Reader
Agilent BioTek Synergy H1 Hybrid Multi-Mode Reader
Agilent BioTek Synergy HTX Multi-Mode Reader
Agilent BioTek Synergy LX Multi-Mode Reader
Agilent BioTek Epoch 2 Microplate Spectrophotometer
Agilent BioTek Epoch Microplate Spectrophotometer
Agilent BioTek ELx808 Absorbance Reader
Agilent BioTek 800 TS Absorbance Reader
Agilent BioTek EL406 Washer Dispenser
Agilent BioTek 405 TS Washer
Agilent BioTek 405 LS Washer
Agilent BioTek 50 TS Washer
Agilent BioTek MultiFlo FX Multi-Mode Dispenser

For Research Use Only. Not for use in diagnostic procedures.

RA44216.3566203704