ELISA and Related Immunoassays
ELISA (Enzyme-Linked-Immunosorbent-Assay) is a very common, robust technique for detecting various analytes. The ELISA technique causes formation of specific immune complexes that can be measured. There are wash and no-wash immunoassays using traditional enzyme-linked technologies with colorimetric, fluorometric or luminometric detection, TR-FRET or HTRF, AlphaScreen and AlphaLISA. ELISAs are typically run in 96- to 1536-well microplates, but can also be performed in micro-volumes using specialized very low volume plates like the Take3™ Micro-Volume Plate. The basic “building blocks” of an ELISA reaction are:
Application Notes:
- Automation of a Novel Cell-Based ELISA for Cell Signaling Pathway Analysis
- Concentration and Detection of Low Levels of Escherichia coli O157:H7, Listeria monocytogenes 4b, and Salmonella enterica Typhimurium in High Organic Load Lettuce Wash
- Using EL406™ Combination Washer Dispenser to Semi-automate a Competitive ELISA for Melamine - Semi-Automated Melamine Quantitation
Learn more about ELISA and Related Immunoassays – see our Applications and technical documentation in Resources.
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- Cytation 5 Cell Imaging Multi-Mode Reader
- Cytation 1 Cell Imaging Multi-Mode Reader
- Synergy Neo2 Hybrid Multi-Mode Reader
- Synergy H1 Hybrid Multi-Mode Reader
- Synergy HTX Multi-Mode Reader
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- Epoch 2 Microplate Spectrophotometer
- Epoch Microplate Spectrophotometer
- ELx808 Absorbance Reader
- 800 TS Absorbance Reader
- EL406 Washer Dispenser
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- 50 TS Washer
- MultiFlo FX Multi-Mode Dispenser