ELISA - BioTek Applications

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  Vollautomatisierte Cell Imager
  
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  Cytation C10 Confocal Imaging Reader
  Multi-Detektions-Reader für Cell Imaging
  
  Cytation 7 Multi-Detektions-Reader für Cell Imaging
  Cytation 5 Multi-Detektions-Reader für Cell Imaging
  Cytation 1 Multi-Detektions-Reader für Cell Imaging
  Multi-Plate Live Cell Imaging System
  
  BioSpa Lebendzell-Plattform
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  Peripheriegeräte
  
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- Detektion
  Multi-Detektions-Reader
  
  Synergy Neo2 Multi-Detektions-Reader mit Hybrid-Technologie
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  Mikroplatten-Reader
  
  Epoch 2 Mikroplatten-Spektralphotometer
  Epoch Mikroplatten-Spektralphotometer
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  Peripheriegeräte
  
  Gaskontroll-Modul
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  Multi-Detektions-Reader
  
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  Washer/Dispenser
  
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  Mikroplatten-Washer
  
  405 TS Mikroplatten-Washer
  405 LS Mikroplatten-Washer
  ELx405 Mikroplatten-Washer
  50 TS Mikroplatten-Washer
  Mikroplatten-Dispenser
  
  MultiFlo FX Multimode-Dispenser
  MicroFill Mikroplatten-Dispenser
  Produktübersicht
  
  Washer
  
  Dispenser
- Automation
  Automation
  
  BioSpa 8 Vollautomatisierter Inkubator
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  BioStack Modelle
- Software
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  Software Apps
  
  Scratch Assay App
  Quantitative Assay Apps
  Cell Count & Viability App
  LogPhase 600 App
  Produktübersicht
  
  Gen5 Datenanalyse-Software
- Buy Cell Culture Microplates
  Sourcing your microplates shouldn't be an added task in preparing your cell-based workflows. Agilent now offers cell culture and imaging microplates so you can complete your workflows seamlessly.
  
  Click here to request your free sample.
- Technologien
  Multi-Mode Detection Technologies
  
  Absorbance
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  TR-FRET
  Imager & Microplate Reader Technologies
  
  4-Zone Incubation and Condensation Control
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  Hybrid Technology
  Laser Autofocus
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  Monochromatoren mit variabler Bandbreite
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  AMX Technology
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- Angebotsanfrage
  
  Nutzen Sie bitte den folgenden Produktkonfigurator zur Auswahl passender Instrument-Modelle.
Peripheriegeräte
- Peripheriegeräte
  BioTek bietet eine Vielzahl an Peripheriegeräten und Zubehörteilen zur Erweiterung der Funktionsvielfalt und Optimierung von Arbeitsabläufen. Die Peripheriegeräte sind mit vielen verschiedenen Produkten kompatibel. Eine vollständige Übersicht finden Sie hier.
- Zubehör
  Um Ihren BioTek Imager, Mikroplatten-Reader, Liquid Handling- oder Automationssystem zu vervollständigen oder zu optimieren, bieten wir eine breite Palette an Zubehör, das von Schläuchen und Flaschen bis hin zu Imaging Filter Cubes reicht. Schauen Sie in unserer Zubehördatenbank nach Zubehör, das mit Ihrem Gerät kompatibel ist. Suchen Sie anhand von Schlagwort, Teilenummer, Produkt oder Zubehör.
- Starter Kits
  BioTeks Starter Kits enthalten alles, was Sie benötigen, um die neusten Assays schnell und nach nur kurzem Training anzuwenden. Die Kits bieten Hardware, Software, Verbrauchsmaterialien und Reagenzien in einem praktischen Set.
Applikationen
- Cell Culture QC
  
  Automated imaging tools can provide valuable information for improving routine cell culturing techniques and increasing the effectiveness and reproducibility of downstream cell-based assays. Conventional methods for evaluating cell culturing techniques and assay design consist of manual inspection of a small subset of the cell population at random locations and time points.
- Cell Health and Viability
  
  Cell health and viability measurements provide essential insight into a broad range of biological processes and treatment responses. Diverse assay formats and reagents have been developed that measure specific aspects of cell viability corresponding to particular cellular response pathways and mechanisms of injury.
- Cell Metabolism
  
  Cellular metabolism comprises a number of biochemical reactions that occur in concert within the cells of living organisms. These orchestrated reactions, better described as metabolic pathways allow organisms to function and respond to environmental changes. Agilent cell metabolism assays detect discrete changes in cell bioenergetics in real time, providing a window into the critical functions that provide ATP, the energy that cells need for activation, signaling, proliferation, and biosynthesis.
- Zellmigration und Invasion
  
  Cell migration and invasion are live cell kinetic assays useful in oncology research. The principle difference between migration and invasion is that migration refers to normal cell movement; whereas invasion describes cells actively invading surrounding tissue. It can do this under the influence of a chemoattractant gradient and secretion of proteases. There are numerous methods for performing these assays including scratch assays performed on a monolayer of cells adhered to plasticware (microplates or culture inserts) or using 3D cell culture models.
- Developmental Biology
  A macro trend in drug discovery is the use of complex models of disease early in preclinical studies. An approach following this viewpoint is the use of whole organism models involving living invertebrates and vertebrates such as nematodes and zebrafish conducive to drug screening methods.
- Histology and Slide Scanning
  Histology is typically performed on fixed tissue samples using upright microscopes equipped with oculars. Cytation™ and Lionheart™ FX provide automated digital imaging of these samples using color brightfield microscopy. Low to high magnifications from 1.25x to 60x (100x with Lionheart FX) can be combined with Cytation Gen5™ image processing features such as image stitching to increase field of view.
- Immunology
  The immune system relies on diverse mechanisms working in concert to defend the host from infection and to identify and remove aberrant or damaged cells. Dysregulation of the immune system can lead to a variety of disease conditions, including autoimmune disorders, allergies, and cancer. Additionally, the immune system is a critical consideration for vaccine development and cell, tissue and organ transplants.
- Immuno-Oncology
  While harnessing the patient’s immune system to fight cancer is not a new concept, there have been significant strides in the past decade to more effectively direct and activate components of the immune system and counter the measures certain tumors adopt to shield itself from immune attack. These include immunotherapeutic drugs based on directing immune cells to tumors based on specific antigens they present, PD-1 checkpoint inhibition and the ex vivo manipulation of the patient’s own immune cells to better drive an immune response to cancer.
- Mikrobiologie
  Since the advent of a lens system that enabled the visualization of bacteria and protists in 1671, the microscope remains a ubiquitous tool for the scientific study of live and fixed microorganisms. Investigating the structure, function, and typing of the highly diverse array of microorganisms spans heterogeneous applications including, but not limited to, the elucidation, diagnosis, and treatment of human and plant disease, food and water safety testing, vaccine development, investigation and production of industrial lipases, protein transfection to mammalian cells, environmental health and impact appraisal, horticultural and agricultural soil assessment, and exploration of alternative fuel sources.
- Molecular Biology and Biochemical
  Biochemical assays run the gamut from routine ELISAs to micro-volume nucleic acid and protein quantification. Endpoint and kinetic, single- and multi-wavelength assays are essential experimental tools. BioTek’s multi-mode readers have the flexibility to accommodate a very broad range of applications with superior performance.
- Rapid Cell Signaling
  G-protein coupled, seven-transmembrane (7-TM) receptors (GPCRs) represent large, diverse gene families found in fungi, plants and animals. The GPCR family of receptors is involved primarily with internalizing extracellular environmental signals via interaction with intracellular G-proteins. GPCR ligands and modulators range from peptide and non-peptide molecules to chemokines, nucleosides, growth factors and light.
- Stem Cell Research
  Research into the reprogramming of somatic cells into pluripotent stem cells received the Nobel Prize in 2012. The long term importance of this research resides in the use of stem cells for regenerative therapies; for the short term, however these cells represent a potential unlimited source of highly physiologically relevant cells, akin to human primary cells, that can be used as drug discovery reagents.
- Virology
  Research into the diverse aspects of virology are made possible by a broad range of applications and assay formats, from relatively simple methods for viral detection to those designed to elucidate complex immune responses and pathological processes. BioTek instruments provide versatile platforms for conducting viral research, with optimized imaging- and plate reader-based solutions, as well as liquid handling and automation tools for increased assay throughput and reproducibility.
Quellen
- Gefragte Applikationen
  
  In BioTeks Applikationshinweisen finden Sie die aktuellsten wissenschaftlichen Inhalte bezogen auf die Anwendungsmöglichkeiten unserer gesamten Produktpalette. Suchen Sie nach Themen, Detektionsmethoden und Produkten oder nutzen Sie einfach unsere Stichwortsuche, um hilfreiche Applikationshinweise zu erhalten.
- Applikations-Mitteilungen
  
  Die Applikations-Mitteilungen von BioTek enthalten kurze, prägnante Beschreibungen aktueller Anwendungen, die mit den Life Science Produkten von BioTek möglich sind.
- App Guides
  
  Viele Anwendungen sind einfach und bedürfen keiner weiteren Erklärungen. Handelt es sich jedoch um komplexe Abläufe sind Best Practice und Tipps & Tricks sehr hilfreich. Hier bietet Ihnen BioTek detaillierte Informationen zu einer Vielzahl an Themen.
- Journale/Publikationen
  Stöbern Sie in unserer Datenbank nach wissenschaftlichen Artikeln die von BioTeks Wissenschaftlern in führenden Fachzeitschriften veröffentlicht wurden.
- Poster
  In über 170 wissenschaftlichen Postern präsentiert Ihnen unser Applikationsteam seine aktuellsten Forschungsergebnisse und Applikationsmöglichkeiten unserer Life Science Produkte. Suchen Sie hier nach interessanten Themen und Produkten.
- Technische Hinweise
  
  Die technischen Hinweise veranschaulichen produktspezifische Eigenschaften und gängige Anwendungsmöglichkeiten unserer Mikroplatten-basierten Produkte in Ihrer täglichen Versuchsplanung.
- Graphische Präsentationen
  BioTeks graphische Präsentationen sind kurze, animierte Präsentationen die die Schrittabfolge bei der Verarbeitung einzelner Imaging- und Multi-Detektions-Anwendungen beschreiben.
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- Literatur
  
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- Videos
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- Kommende Webinare
  In unseren Webinar-Präsentationen geben wir Ihnen hilfreiche Informationen und nützliche Tipps zu den aktuellsten Mikroplatten-Anwendungen. Registrieren Sie sich bereits heute und erfahren Sie mehr über zukünftige Themen.
Technische Unterstützung
- Vertriebsbüros
  BioTek's highly trained sales and service professionals are experts in life science instrumentation. They will guide you through your decision process and help you select the proper equipment for your application, assist with installation in your lab, and train your staff on the use of the instrumentation.
- Field Applications Scientists
  BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software.
- Technische Unterstützung
  The BioTek Global Technical Support Center (GTS) is your Help Desk for all questions related to BioTek product use, maintenance, troubleshooting support and other frequently asked questions. Normal GTS hours are weekdays from 8:30 A.M. - 5:30 P.M Eastern Standard Time, excluding holidays.
- Weltweiter Service
  BioTek Instrument Service and Repair is available worldwide at several of our International Service Centers. Field Service is also available for many customers throughout the world.
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Über uns
- News
  BioTek's Cytation 7 Cell Imaging Multi-Mode Reader Named ‘Best New Drug Discovery & Development Product’ at Scientists’ Choice Awards
  May 5, 2021 - BioTek Instruments is pleased to announce that the Cytation 7 Cell Imaging Multi-Mode Reader was awarded ‘Best New Drug Discovery & Development Product’ of 2020. The honor was voted upon by SelectScience members across the world and celebrated at the 2021 Scientists’ Choice Awards online awards ceremony. Weiterlesen
  
  BioTek Announces New Cytation C10 Confocal Imaging Reader
  February 2, 2021 - BioTek Instruments, a part of Agilent, is excited to unveil the Cytation C10, an advanced, automated Confocal Imaging Reader. This versatile and multi-functional automated system is ideal for research laboratories and core facilities looking to increase productivity while reaping high-quality qualitative and quantitative data to support cell-based research. Weiterlesen
  
  BioTek Receives Patent for Automated Media Exchange (AMX) Module for the MultiFlo FX Multi-Mode Dispenser
  January 28, 2021 - BioTek Instruments, an Agilent Technologies, Inc., company, announces that it was granted a patent by the United States Patent and Trademark Office for the Automated Media Exchange (AMX) module for the MultiFlo FX Multi-Mode Dispenser. In cell biology workflows, the automated MultiFlo FX with AMX module enables hands-free, gentle, and highly consistent media exchanges and washing in 96- and 384-well microplate formats without disturbing sensitive cells. This promotes viability and long-term differentiation in 3-dimensional (3D) cell culture models as well as effective maintenance of loosely adherent 2-dimensional (2D) cell cultures. Weiterlesen
  Weitere News
- Veranstaltungen
  ISSCR 2021 - Virtual
  
  June 21, 2021
  
  BioTek instrumentation will be on display by BioTek U.S.
  
  Online
  
  BIO Asia-Taiwan Exhibition 2021
  
  July 22 - 25, 2021
  
  Taipei Nangang Exhibition Center
  BioTek instrumentation will be on display by BioTek Taiwan.
  
  Taipei City
  Taiwan
  
  Labvolution 2021 - Hannover
  
  September 7 - 9, 2021
  
  Hannover Messe
  BioTek instrumentation will be on display by BioTek Germany.
  
  Hannover
  Germany
  
  Weitere Veranstaltungen
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  At BioTek we feel passionately and genuinely that our employees are our greatest asset. We continue to build our already impressive team having doubled our global workforce in the last five years. With our headquarters in the beautiful Green Mountains of Vermont, USA, we also have regional offices around the world.
  
  If you're interested in becoming part of our amazing team, check out our list of job openings today!
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- Karriere
  At BioTek we feel passionately and genuinely that our employees are our greatest asset. We continue to build our already impressive team having doubled our global workforce in the last five years. With our headquarters in the beautiful Green Mountains of Vermont, USA, we also have regional offices around the world.
  
  If you're interested in becoming part of our amazing team, check out our list of job openings today!
- Kontakt
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The enzyme linked immunosorbent assay (ELISA) is a very common, robust technique for detecting various analytes. It spawned a broad range of immunoassays that utilize either wash or no-wash workflows using colorimetric, fluorometric, luminometric, TRF or TR-FRET, AlphaScreen and AlphaLISA detection technologies. Some of these latter technologies utilize low volume analysis in 1536-well plates, yet ELISA performed in 96-well microplates remains the most used immunoassay technique. The ELISA technique causes formation of specific immune complexes that are easily measured using the Agilent BioTek single or multimode microplate readers. The basic building blocks of an ELISA reaction are:

ELISA process

Application Notes

Technical Notes

Benchtop ELISA Automation with BenchCel Microplate Handler

Publications

Comparison of plaque reduction neutralisation test (PRNT) and measles virus-specific IgG ELISA for assessing immunogenicity of measles vaccination

Application Bulletins

Detection of HTLV I and II Antibodies using Absorbance based ELISA

Learn more about ELISA – see our applications and technical documentation in Resources.

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For Research Use Only. Not for use in diagnostic procedures.

RA44216.3566203704