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ELISA and Related Immunoassays

ELISA (Enzyme-Linked-Immunosorbent-Assay) is a very common, robust technique for detecting various analytes. The ELISA technique causes formation of specific immune complexes that can be measured. There are wash and no-wash immunoassays using traditional enzyme-linked technologies with colorimetric, fluorometric or luminometric detection, TR-FRET or HTRF, AlphaScreen and AlphaLISA. ELISAs are typically run in 96- to 1536-well microplates, but can also be performed in micro-volumes using specialized very low volume plates like the Take3™ Micro-Volume Plate. The basic “building blocks” of an ELISA reaction are:

ELISA process

 

Application Notes:

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