Confluence measurements provide valuable information on the extent that cell populations have changed over time. This measurement is routinely determined prior to splitting cells in tissue culture or for assays determining cell proliferation where cell counting may be problematic (i.e. cell types that tend to clump). BioTek’s Lionheart FX and Cytation imaging systems, along with Gen5 software, can automate the analysis across any number of labware, from culture flasks, petri dishes and low density microplates (6- and 12-well microplates) suitable for tissue culture to higher density microplates used for cell-based assays.


NIH3T3 treated with 0.1 µM doxorubicin
Watch The Video - Confluence 1
NIH3T3 treated with vehicle
Watch The Video




Application Notes:

Visual Abstracts (.mp4):

BioTek’s Visual Abstracts are brief animated presentations describing the Augmented Microcopy workflow of a specific application.

Cell Proliferation
Cell Proliferation - Label-free Confluence



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