Phenotypic assays are quantitative assays of cellular structure and function using digital widefield microscopy using both fixed and live cells under environment control. They are routinely used to assess whether a compound or drug produces a desired effect, independent of any defined target. To measure phenotypic assays, Gen5 can analyze multiple cellular parameters simultaneously including size, shape, area, and intensity. This provides a comprehensive picture of individual cell phenotypes and entire population phenotypes that allows a researcher to make an educated decision. Assays of this type typically use culture inserts and 6- to 384-well microplates.
Phase contrast monitoring of MDA-MB-231 cells undergoing oridonin-induced apoptosis at 0, 6 and 20 hrs.
- Phenotypic and Epigenetic Mechanism of Action Determinations of Histone Methylase and Demethylase Inhibitors using Digital Widefield Microscopy
- Induction and Inhibition Studies of Hypoxia and Oxidative Stress in Immortalized Keratinocytes
- Label-Free Phenotype MicroArrays™ Analysis of Cellular Energetics and Apoptotic Activity using Microplate Reading and Phase Contrast Imaging
Learn more about the broad range of phenotypic assay applications in our Resources page.