Scratch Assay Starter Kit
for Wound Healing and Cell Migration Assays

BioTek’s Scratch Assay Starter Kit includes everything a researcher needs to implement automated, kinetic cell migration and scratch wound assays, in conjunction with a BioTek Lionheart™ Automated Microscope or Cytation™ Cell Imaging Multi-Mode Reader.


Each Scratch Assay Starter Kit includes:

• BioTek’s AutoScratch™ Wound Making Tool to automatically create repeatable scratch wounds
• The Scratch Assay App software: Automatically calculates key statistics including wound width, % confluence and maximum healing rate
• Sample packages of 24-well and 96-well microplates
• Reagents to ensure the best scratch performance, assay repeatability and easy maintenance

• PN 02682 - Case of 100 (individually wrapped) Corning® Costar® 24-well Clear TC-Treated Plates - flat bottom, polystyrene, sterile, lid
• PN 02683 - Case of 100 (20 packs of 5) Corning® 96-well Clear TC-Treated Plates - flat bottom, polystyrene, sterile, lid
• PN 1750006 - Set of 8 spare scratch pins for AutoScratch Wound Making Tool
• PN 1753008  - Cleaning reagents for AutoScratch Wound Making Tool

• Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays

Visual Abstracts:

Related Videos:

This is a tutorial video about optimizing your microplate-based scratch migration assays by effectively washing samples after scratches have been formed. Post-scratch washing is an important step in the wound healing assay protocol, whether you are generating your scratches manually, or using the fully automated BioTek AutoScratch Wound Making Tool to efficiently create uniform scratches in either 24- or 96-well microplates.

Upon completion of automated wound creation using the AutoScratch tool, automated plate washing can be achieved using the MultiFlo FX to remove cells dislodged from the plate bottom. A series of three plate washes are completed in a sterile environment followed by media and/or inhibitor addition prior to performing cell migration studies. Learn more at www.biotek.com/multiflofx.  

FEATURES

Simple installation and implementation

The Scratch Assay Starter kit automates cell migration and scratch wound healing assays, from scratch wound creation to image and kinetic cell migration analysis. The Kit automates plate setup, kinetic image capture and processing, image analysis and export of results, videos and graphs.

Consistent, repeatable wounds

Manual methods for creating scratch wounds are prone to error and inconsistency. AutoScratch Wound Making Tool automates the process for 24- and 96-well plates to ensure high quality, consistent wounds across the plate.

Pre-defined protocols

Start with a simple, pre-defined protocol and customize to your assay requirements. Temperature, assay runtime, kinetic interval and other settings are accessed through easy navigation. Automated exposure finds the optimal exposure for your samples.

Automated calculations
 

After kinetic image capture, the Scratch Assay App automatically calculates % confluence, wound width and maximum healing rate (max V).

 

Automated, multi-step cleaning and decontamination
 

Keep the scratch pins clean and prevent carryover, using the automated cleaning and decontamination routines. AutoScratch has built-in cleaning troughs, and the Kit includes cleaning reagents for convenience.