Synergy™ Neo2 Multi-Mode Microplate Reader is designed for the screening laboratory, with speed and ultra-high performance. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF and up to 4 PMTs provide ultra-fast measurements with excellent results. For walk-away automation and high throughput, BioStack Neo is the fastest on the market, and the barcode-labeled filter cubes help streamline workflows and limit errors. Synergy Neo2 features BioTek’s patented Hybrid Technology™, with its independent optical paths that ensure uncompromised performance in all detection modes. Advanced environmental controls, including CO2/O2 control, incubation to 65 °C and variable shaking support live cell assays; cell-based detection is optimized with direct bottom illumination. Powerful Gen5 Software offers complete reader control, powerful data analysis, and automation and LIMS integration.
- Patented Hybrid Technology™ with independent filter and monochromator-based optics
- Ultra-fast plate processing speeds with multiple PMT detectors
- Two lasers: laser for TRF, TR-FRET and laser-based Alpha detection
- Variable bandwidth scientific quad monochromators for optimal sensitivity and flexibility
- Controlled environment and direct bottom detection for live cell assays
- Gen5 software for complete control and automation and LIMS integration into automation and LIMS
What's New
The TRF laser provides ultra-fast measurement speed, along with optimal sensitivity for critical screening applications like TRF, and TR-FRET. Laser-based detection ensures the best performance for Alpha assays.Anwendungen
Synergy Neo2 is well suited for many applications requiring a variety of detection modes. Here we list some of the most important applications for this fast, high performance microplate reader.

hTNFα analyte titrations

Nucleic acid quantification using PicoGreen®

Micro-volume (2 µL) quantification of up to 48 samples with Take3 Plate
These are just a few examples of the many important applications for Synergy Neo2. Visit our Applications page to see more.
BioTek Field Applications Scientists (FAS) and Product Specialists provide unparalleled scientific support, assisting with experimental planning and assay optimization on BioTek’s instrumentation and software. Learn how they can help move your science forward… faster!
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Patented Hybrid Technology

Hybrid Technology offers many benefits for screening and other multi-mode workflows, including the flexibility to easily select a discrete wavelength and perform spectral scans for excitation or emission peaks. Filter-based fluorescence provides fast wavelength switching and excellent sensitivity.
Variable bandwidth monochromators
Synergy Neo2 has quadruple scientific monochromators, with the fluorescence module offering variable bandwidths from 3 nm to 50 nm, selectable in 1 nm increments. Variable bandwidths allow you to optimize detection conditions for fluorophores that have unique excitation or emission parameters, for example, a narrow Stokes shift, to provide better performance than conventional monochromators.

Live-cell assay optimized
Temperature control to 65 °C, plus linear and orbital shaking and an available gas controller module optimize the environment in Synergy Neo2 for live cell assays. The dedicted filter-based bottom optical path directly illuminates the plate bottom, increasing the sensitivity of live cell measurements, while a dual reagent injector provides quick inject/image capability for observing fast reactions.
Gen5 Data Analysis Software
Gen5 Microplate Reader and Imager Software doesn’t compromise on usability or data analysis performance – protocol creation is intuitive and flexible, and the data analysis capability rivals standalone packages. For the GxP lab, Gen5 Secure software offers features to help comply with 21 CFR Part 11, including electronic signature, data security and multiple level permissions.

Customer Spotlight

23-January-19
Most organisms have circadian clocks, which regulate 24- hour rhythms in a wide variety of activities and behaviors. In humans, the circadian clock regulates sleep onset and influences how much sleep you need. Not surprisingly, changes to these clock factors influence sleep, but also to a wide variety of disorders such as metabolic syndrome, cardiovascular disease, and even cancer. Dr. John Hogenesch, Ohio Eminent Scholar; Professor of Pediatrics in the Divisions of Human Genetics and Immunobiology and the Perinatal Institute; and Deputy Director, Center for Chronobiology at the Cincinnati Children's Hospital Medical Center, in Cincinnati, Ohio, is one of the world’s foremost experts in the molecular mechanisms of circadian rhythms in mammals. His research centers around understanding how biological clocks work and leveraging them to improve human health.