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Spectrophotometric Nucleic Acid Quantification in Microplates and Micro-Volumes
June 27, 2014
Why Quantify Nucleic Acids?
Nucleic acids are the building blocks of life in all living things, from plants and animals to bacteria and viruses. In research, it’s important to quantify RNA and DNA prior to downstream processes like sequencing, restriction enzyme digestions and ligations, PCR and qPCR along with many other applications. Along with determining nucleic acid concentrations, it’s also important to calculate the ratio of nucleic acid to protein to ascertain purity before using the sample in downstream applications.
There are fluorometric and spectrophotometric methods for nucleic acid quantification. The former is used for applications requiring high sensitivity due to minute available amounts of nucleic acid; the latter is more conventionally used from common nucleic acid extraction procedures. In this application guide, we’ll discuss in detail spectrophotometric methodology as performed in microplate instrumentation and in specialized micro-volume accessories.
This Application Guide covers:
- Absorbance Quantification Methods
- Cuvettes and other Sample Vessel Types
- Path Length Correction and Nucleic Acid Quantification
- Nucleic Acid Purity Assessment
- Best Practices
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