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Automated Cell Dispensing into 1536-Well Microplates for HTS ScreeningDownload
January 26, 2010
Authors: Paul Held, Brad Larson, and Peter Banks, BioTek Instruments, Inc., Winooski, VT, USA
Today’s HTS demands have moved screening assays towards high well density plates as well as placed more emphasis on cell-based assays in lieu of the conventional biochemical determinations. High density plates allow more samples to be assayed simultaneously, conserve reagents and lower assay costs. The use of 1536-well microplates for cell-based assays requires the use of accurate and reliable automation in order to dispense uniform numbers of cells to each microplate well in a volume of a few microliters. Here we describe the performance of the MicroFlo Select peristaltic pump dispenser to dispense different cell lines into 1536-well microplates. As with any cellbased experiment, providing uniform numbers of viable cells is paramount to successful experiments. CHO-M1 cells (200 cells/ μL) were dispensed into 1536-well microplates (4 μL/well) and cell uniformity as measured by the luminescent determination of ATP using CellTiter-Glo assay kits from Promega. Luminescent signal for wells not containing cells averaged 10, while those wells with 800 cells averaged 23210. The %CV for the entire plate was 8.06% with a Z’ =0.753 when compared to the plate with media only. The average %CV for the individual rows of the plate was 5.33%, while that of the columns was 7.84%. Details regarding work fl ow and instrument performance will be provided.