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Automation of a Microplate Cell-based Assay to Measure Activity of the Histamine H1 G-protein-coupled Receptor Using A Novel 3-D Cell Culture TechniqueDownload
January 22, 2010
Authors: Brad Larson, Peter Banks, BioTek Instruments, Inc., Winooski, VT; Diana Berry, Brad Justice, Global Cell Solutions Inc., Charlottesville, VA, USA; Ronald Herzig, Invitrogen Corporation, Madison, WI, USA
Cell-based assays using recombinant drug targets expressed in immortalized cell lines are today used more frequently than their biochemical counterparts in drug screening. Current cell culture methods use two-dimensional (2-D) techniques where cells attach to the microplate surface in a single monolayer. In order to maintain cell lines, complicated and time-consuming methods are used to trypsinize cells off of their growth surface, deactivate and remove the trypsin, split, and then replace cells into a new flask. This becomes increasingly diffi cult as scale-up is required for primary and secondary screens.