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Utility of Hybrid Multi-Mode Readers using Both Monochromator- and Filter-Based Detection Capabilities as Demonstrated with FP, TR-FRET, and Fluorescence Intensity Assays for the Measurement of ADP AccumulationDownload
January 26, 2010
Authors: Brad Larson, Xavier Amouretti, Peter Banks, BioTek Instruments, Inc., Winooski, VT, USA; Meera Kumar, John Majer, BellBrook Labs, Madison, WI, USA
• The Synergy™ H4 Hybrid Microplate Reader incorporates BioTek’s patent-pending Hybrid Technology™ which combines two fluorescence detection systems, monochromator-based and filter-based, into one unit.
• The monochromator system allows multiple fluors to be tested during assay development without the need to switch out filters. Fluors requiring higher spectral resolution, or possessing narrow Stokes shift can easily be scanned using the variable (9-20 nm) bandpass capability in the system.
• The sensitivity of the instrument’s deep blocking filters and dichroic mirrors provide quality data using short read times with all fluorescent detection modes.
• Data generated with the Transcreener® ADP2 Assay, as well as the results of the spectral scan, demonstrate the ease that the assay’s detection modules can be read, as well as the ability of the reader to yield high quality information using either detection system.