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Versatile, Cost-effective Automation of Avian Influenza and Mycoplasma Gallisepticum-Synoviae ELISAsDownload
January 26, 2010
Authors: Wendy Goodrich, Jason Greene, BioTek Instruments, Inc., Winooski, VT, USA; Matt Durgin, IDEXX Laboratories, Westbrook, ME, USA
Highly pathogenic Avian Influenza (AI) subtypes are viruses that cause extensive loss of fowl and poultry stocks, and in the case of the H5N1 subtype, can infect humans creating the potential for a global flu pandemic. Mycoplasma Gallisepticum-Synoviae (Mg/Ms) infections also spread rapidly in poultry populations causing widespread loss of production and stock. Ensuring the health of the global agricultural economy and minimizing the probability of a worldwide flu pandemic is placing growing demands on laboratories that routinely test poultry and livestock samples. These demands can be met with carefully planned automation. Two of the biggest obstacles in designing successful automated assay throughput models are managing fluid overages within the confines of the reagent volumes supplied by kits, and processing batches in optimal quantities while staying within the incubation windows of the assay steps. Here we demonstrate the workflow versatility and cost-effective reagent usage of EL406 Combination Microplate Washer/Dispenser using the IDEXX’s Mycoplasma Gallisepticum-Synoviae ELISA indirect format kit and Avian Influenza Multi- Species ELISA blocking format kit. Data generated for both a small batch model composed of multiple runs of less than five plates, and a larger batch model integrating the BioStackTM robotic microplate handler for running batches of five or more plates, demonstrate reliable, reproducible results with an expected level of inter- and intra-batch variability for both assays while offering workflow versatility that optimizes both throughput and reagent usage.