Area scan (31 x 31 points) on full 24-well microplate with adherent cell layer labeled with calcein

Gen5 version required: 2.0 or higher

One of the problems when reading cell assays in microplates is that the cell population is often not homogeneous at the bottom of the wells and measuring only at the center of the well leads to inaccuracies. The area scanning read mode allows correcting for this artifact by providing an averaged result over the entire area of the well.  This sample file demonstrates Gen5’s area scanning feature on a 24 well microplate with various concentrations of cells. Cells are labeled with Calcein after a 48-hour incubation time.

 
31x31 area scan of wells of a 24-well plate with cells labeled with Calcein