Gen5 version required: 1.0 or higher
Basis for the Assay:
Reporter genes, such as luciferase, are important tools for studying gene expression. The use of reporter genes allows the in vitro and in vivo measurement of gene expression from virtually any endogenous genetic control element. Luciferase enzyme and its subsequent luminescent reaction is often the gene reporter of choice for many experimental conditions. “Glow” assays are typically less sensitive than “Flash” assays (see Flash Luciferase Assay), but don’t require automated injection and have a higher throughput.
This protocol is an end-point luminescence measurement integrating each well for 1 second.
The experiment data file does not contain any data.
Plate configuration contains samples in duplicate and a Blank well in duplicate. It may have to be updated with more specific information (e.g., standards to generate a calibration curve, controls).
The report provides:
1. The plate layout and raw data in a matrix format
2. Statistics on replicates