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Counting Bacilli in Agar Mounted Soil Samples

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July 03, 2019

The bacillus genus of bacteria contains common soil and intestinal microbes. Fluorescent staining is a useful way to study these organisms using a microscope, but bacilli cells may populate more than one focal plane in mounting media like agar. Combining images from multiple focal planes into a final z-projection can result in more accurate counts.

 


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