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Measuring Apoptosis in Zebrafish Treated with Ethanol

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March 26, 2018

Zebrafish embryos are treated with ethanol during the first 24 hours of development and the effects of ethanol treatment on cell death is assessed using acridine orange staining (green emission). Embryos were imaged in 96-well round bottom plates with a 2x objective as z-stacks in the bright field and GFP channels.