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Quantifying Poly-Glutamine Aggregates in C. Elegans Using vivoChip

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August 24, 2018

C. elegans has emerged as a tool for whole organism based high-throughput screening as they model complex human diseases that can not be easily reproduced in vitro. Here, we use a model of Huntington’s disease which consists of poly-glutamine aggregation (PolyQ35:YFP). C. elegans were loaded onto the vivoChip™ (Newormics) and imaged in the YFP channel. Outlines of the worms were identified using Gen5™, and the secondary mask function was used to count the aggregates per worm.

Quantifying Poly-Glutamine Aggregaters in C. Elegans Using vivoChip
 


 

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