Quellen - Webinare
ELISA: Best Practices and Troubleshooting - On Demand
Related Products: 405 LS Washer, 405 TS Washer, 50 TS Washer , 800 TS Reader für Absorptionsmessungen , BioSpa 8 Vollautomatisierter Inkubator, BioSpa Lebendzell-Plattform , BioStack Mikroplatten-Stacker , Cytation Multi-Mode, EL406 Washer/Dispenser-Kombination, ELx405 Select Deep Well-Washer , Gen5 Funktionen , Liquid Handling Control™ Software , Synergy H1 Multi-Detektions-Reader , Synergy HTX Multi-Detektions-Reader , Synergy Neo2 Multi-Detektions-Reader
September 20, 2017
Presenter: Dr. Paul Held - Applications Laboratory Manager, BioTek Instruments
Enzyme-linked immunosorbent assay (ELISA) is an antibody-based reaction that allows the detection and quantitation of numerous analytes with the same basic assay process. The reaction uses a solid surface, typically a microplate, to immobilize the analyte. Once immobilized, unwanted materials can be removed by washing and the remaining analyte quantitated. The assay technology uses antibodies in conjunction with enzyme-catalyzed amplification to provide high specificity and high sensitivity. Here we will describe the basic process steps of ELISA, best laboratory practices along with troubleshooting tips for common problems. In addition, some of the devices and instrumentation used with this assay technology will be described.