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BioTek Resources for Agilent MitoXpress® Xtra Oxygen Consumption Assay
DownloadRelated Products: Cytation 1 Multi-Detektions-Reader für Cell Imaging , Cytation 5 Multi-Detektions-Reader für Cell Imaging , Synergy H1 Multi-Detektions-Reader , Synergy Neo2 Multi-Detektions-Reader mit Hybrid-Technologie
June 27, 2018
Related Sample File: AgilentX02, MitoXpress
Introduction
The Agilent MitoXpress® Xtra Oxygen Consumption Assay allows real-time measurement of oxygen consumption of whole cells or isolated mitochondria. BioTek offers a dedicated filter cube assembly, pre-programmed Gen5™ protocol, and user tutorial specifically optimized for use with the lifetime signal acquisition of the assay on the BioTek Cytation™ 1, Cytation 5, Synergy™ H1, or Synergy Neo2 instruments equipped with TRF capability.
Note: The User Tutorial and Data Analysis Protocols are compatible with Gen5 v3.04 and higher software. Existing Gen5 users can upgrade their v3.0x software for free at: https://btresource.force.com/CRC/s/
Figure 1 - Example Data (Cytation 5): Lifetime detection of extracellular oxygen consumption in HepG2 cells (left). Slope is calculated from the linear portion of the curves to calculate relative oxygen consumption rates (OCR) of various cell treatments (right).
Figure 2. A summary of the AgilentpHX.prt procedure, plate layout, and data reduction steps as shown from within Gen5 v3.04 software.
Figure 3. Top level implementation steps for the Agilent MitoXpress Xtra – Oxygen Consumption Assay with the BioTek Cytation 1, Cytation 5, Synergy H1, or Synergy Neo2 instruments. The toolkit is designed for models equipped with Time Resolved Fluorescence (TRF) capability.