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DELFIA ADCC Cytotoxicity Assay reading parameters and % Maximum Cytotoxicity calculations for EC50 value

March 08, 2013

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Related Products: Gen5 for Detection, Synergy H1

red Related Application Note: An Automated DELFIA ADCC Assay Method using a CD16.NK-92 Cell Line


Gen5 software is used to compute % Maximum Cytotoxicity values using the raw fluorescence values and plate layout. A dose response curve is then generated using the cytotoxicity data, and an EC50 value is calculated for the test antibody. Fluorescence measurements indicate the level of target cells being lysed due to antibody and effector cell binding. As shown in this experiment file, increasing cytotoxicity is seen with increased binding and EC50 values are derived from the dose-response curve.
 

DELFIA ADCC Cytotoxicity Assay reading parameters and % Maximum Cytotoxicity calculations for EC50 value
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