Luminescence-based assay using Promega’s GloSensor technology, with dose-response curves and EC50 calculations
June 08, 2011Download
Related Products: Gen5 Funktionen , Synergy Neo2 Multi-Detektions-Reader mit Hybrid-Technologie
Related App Note: Live Cell Biosensor Assay Used To Interrogate GPCRs
Gen5 version required: 2.0 or higher
The GloSensor technology from Promega is based on a biosensor protein expressed from a genetically modified form of luciferase. This biosensor is capable of modulating its luminescence activity dependent on reversible allosteric interaction with ligand allowing live-cell, real-time monitoring of cAMP kinetics and allows investigation of both endogenous and over-expressed GPCR targets.
This file illustrates the reading parameters used for an end-point luminescence GloSensor assay, as well as Gen5’s ability to plot titration curves using a 4-parameter fit and to calculate EC50 values.
Raw data and titration curves with multiple curves overlaid