Cell Proliferation - Label-free Confluence

Related Applications: Cell Proliferation, Confluence, Live Cell Imaging

Cells are seeded into 96-well microplates at 2000 cells per well. Environmental conditions, including temperature (37 °C), gas (5% CO₂) and humidity (90%) are maintained during a five day incubation by a BioSpa™ 8 Automated Incubator. Proliferation or drug-induced reduction in proliferation is detected by label-free cell counting using high contrast brightfield.